Evaluation of a commercial Erns-capture ELISA for detection of BVDV in routine diagnostic cattle serum samples

Table 1 Results from testing for BVDV in sera collected between days 0 and 11 post partum from nine PI calves born after experimentally infected heifers, using a commercial E^rns-capture ELISA (HerdCheck BVDV antigen/Serum Plus, IDEXX Scandinavia AB, Österbybruk, Sweden.) and the indirect immunoperoxidase test (IPX).

PI calf no.	Age (days)	Antibody titre	Virus detection
			IPX	E^rnsELISA^a	(COD)
1	0	<1:10	-	+	(3.32)
	2	<1:10	+	+	(2.96)
	7	<1:10	+	+	(2.70)
2	0	<1:10	+	+	(2.99)
	2	<1:10	+	+	(3.52)
	3	<1:10	+	+	(3.42)
3	0	<1:10	-	+	(3.21)
	2	<1:10	+	+	(3.00)
	7	<1:10	+	+	(2.97)
	11	<1:10	+	+	(2.52)
4	0	<1:10	+	+	(3.33)
5	0	<1:10	-	+	(3.56)
	2	<1:10	+	+	(3.26)
6	0	<1:10	+	+	(3.27)
	2	1:250	-	-	(0.16)
	5	1:250	-	+	(3.29)
	7	1:10	-	+	(3.28)
7	2	1:250	-	+	(0.48)
8	4	1:1250	-	-	(0.29)
	6	1:250	-	+	(1.58)
9	2	1:1250	-	+	(2.92)
	7	1:1250	-	-	(0.04)
	9	1:1250	-	-	(0.07)

^{Calves 1–5 were given colostrum free from BVDV antibodies, and calves 6–9 were given colostrum from their respective antibody positive dams. Samples collected day 0 were taken before intake of colostrum. BVDV antibody titres were determined using a commercial indirect ELISA (SVANOVA Biotech AB, Uppsala, Sweden) in dilutions 1:10–1:1250.}
^a Samples with COD > 0.30 as measured by the Erns ELISA were considered positive

ISSN: 1751-0147