Table 2 Characteristics of gene-specific primers
Gene | Forward primer (from 5′ to 3′) | PCR product size (bp) | NCBI GenBank |
|---|---|---|---|
Reverse primer (from 5′ to 3′) | |||
Reference genes | |||
ATP5G1 | CAGTCACCTTGAGCCGGGCGA | 94 | NM_001025218.1 |
TAGCGCCCCGGTGGTTTGC | |||
ACTB | GACATCCGCAAGCACCTCTA | 205 | NM_001167795 |
ACATCTGCTGGAAGGTGGAC | |||
GAPDH | AGGGGCTCTCCAGAACATCATCC | 446 | AF017079.1 |
TCGCGTGCTCTTGCTGGGGTTGG | |||
GPI | CACGAGCACCGCTCTGACCT | 365 | NM_214330.1 |
CCACTCCGGACACGCTTGCA | |||
RPS9 | GTCGCAAGACTTATGTGACC | 327 | CAA23101 |
AGCTTAAAGACCTGGGTCTG | |||
SDHA | CTACGCCCCCGTCGCAAAGG | 380 | DQ402993 |
AGTTTGCCCCCAGGCGGTTG | |||
Target genes | |||
CCL2 | GGTCCTTGCCCAGCCAGATGC | 170 | NM_214214.1 |
CTGCACAGATCTCCTTGCCCGC | |||
CYP1A1 | CTGCCATCTTCTGCCTTGTA | 314 | NM_214412.1 |
GCTCTGGCCATTAGAGATCA | |||
GPX1 | CTTCGAGAAGTTCCTGGTGG | 232 | NM_214201.1 |
CCTGGACATCAGGTGTTCCT | |||
HMOX1 | AGCTGTTTCTGAGCCTCCAA | 130 | NM_001004027.1 |
CAAGACGGAAACACGAGACA | |||
HP | GTTCGCTATCACTGCCAAAC | 108 | NM_214000.1 |
CAGTTTCTCTCCAGTGACCT | |||
ICAM1 | CGGTGGCAGCCGTGGCTATC | 208 | NM_213816.1 |
TTGATGCAGCCCCGCTCGTC | |||
IL1B | GTTCTCTGAGAAATGGGAGC | 143 | NM_214055.1 |
CTGGTCATCATCACAGAAGG | |||
IL8 | ACTTCCAAACTGGCTGTTGC | 120 | NM_213867.1 |
GGAATGCGTATTTATGCACTGG | |||
IL6 | AGCAAGGAGGTACTGGCAGA | 257 | NM_001252429.1 |
GTGGTGGCTTTGTCTGGATT | |||
NQO1 | CCAGCAGCCCGGCCAATCTG | 160 | NM_001159613.1 |
AGGTCCGACACGGCGACCTC | |||
PRDX6 | GGCCGCATCCGTTTCCACGA | 280 | NM_214408.1 |
ACTGGATGGCAAGGTCCCGACT | |||
SOD1 | TCCATGTCCATCAGTTTGGA | 250 | NM_001190422.1 |
CTGCCCAAGTCATCTGGTTT | |||
SAA | GGCATCATTCCTCAAGGAAG | 168 | NM_001044552.1 |
CTGATCACTTTAGCAGCCCA | |||
TNFα | CATGAGCACTGAGAGCATGA | 170 | NM_214022.1 |
CGATAACTTCGAAGTGCAGT | |||
TXNRD1 | CTTTACCTTATTGCCCGGGT | 162 | NM_214154.2 |
GTTCACCGATTTTGTTGGCC | |||