Table 2 Quantification of Mycobacterium cells by microscopy and by qPCR
From: Quantification of Mycobacterium avium subspecies in pig tissues by real-time quantitative PCR
Strain/sample | Microscopy cells ml-1 | qPCR cell equivalents* of mycobacterial DNA ml-1 |
|---|---|---|
M. avium ATCC 25291 | 1 × 106 | 2 × 106 (n = 2) |
M. avium ATCC 25291 | 1 × 108 | 6 × 107 (n = 2) |
M. avium Austria 9646/4 | 5 × 108 | 2 × 108 |
M. avium Austria 9972/6 | 7 × 109 | 3 × 109 |
M. avium Netherlands 2 | 2 × 109 | 9 × 108 |
M. avium ATCC 25291 + 0.1 g of liver | 1 × 104 | 7 × 103 |
M. avium ATCC 25291 + 0.1 g of liver | 1 × 106 | 7 × 105 |