Skip to main content

Table 1 Characteristics of primers and primer performance data used for qPCR

From: The stress signalling pathway nuclear factor E2-related factor 2 is activated in the liver of sows during lactation

Gene symbol

Forward primer (from 5’ to 3’)

Product size (bp)

NCBI GenBank

Slope

R2#

Efficiency*

M

 

Reverse primer (from 5’ to 3’)

      

Reference genes

RSP9

GTCGCAAGACTTATGTGACC

325

XM_003356050

-0.28

0.999

1.91

0.053

 

AGCTTAAAGACCTGGGTCT

      

ATP5G1

CAGTCACCTTGAGCCGGGCA

94

NM_001025218

-0.30

0.998

1.99

0.054

 

TAGCGCCCCGGTGGTTTGC

      

GSR

AGCGCGATGCCTACGTGAGC

175

AY368271

-0.29

0.997

1.94

0.055

 

GGTACGCCGCCTGTGGCAAT

      

ACTB

GACATCCGCAAGGACCTCTA

205

XM_003124280

-0.32

0.992

2.1

0.064

 

ACATCTGCTGGAAGGTGGAC

      

SHAS2

GAAAAGGCTAACCTACCCTG

218

NM_214053

-0.21

0.996

1.65

0.076

 

TGTTGGACAAGACCAGTTGG

      

Target genes

HP

ACAGATGACAGCTGCCCAAA

188

NM_214000

-0.30

0.997

1.99

 
 

CCGCACACTGCTTCACATTC

      

FGG

GACATCTGTCTCCTACTGGA

375

NM_001244524

-0.29

0.999

1.95

 
 

CATGACACTTGTTCATCCAC

      

CFB

CTCAACGCAAAGACCGCAAA

106

NM_001101824

-0.29

0.998

1.96

 
 

AAATGGGCCTGATGGTCTGG

      

CRP

CCTTTGTCTTCCCCAAAGAG

563

NM_213844

-0.28

0.999

1.91

 
 

CACCTCGCCACTCATTTCAT

      

LBP

ACCGCTCCCCAGTTGGCTTC

406

NM_001128435

-0.29

0.999

1.96

 
 

AGCGCGGCGGACACATTAGT

      

NQO1

CCAGCAGCCCGGCCAATCTG

160

NM_001159613

-0.28

0.997

1.89

 
 

AGGTCCGACACGGCGACCTC

      

TXNRD1

CTTTACCTTATTGCCCGGGT

162

NM_214154

-0.30

0.999

1.98

 
 

GTTCACCGATTTTGTTGGCC

      

UGT1A1

GATCCTTTCCTGCAACGCAT

313

XM_003483776

-0.28

0.996

1.91

 
 

GGAAGGTCATGTGATCTGAG

      

HO-1

AGCTGTTTCTGAGCCTCCAA

130

NM_001004027

-0.30

0.998

1.98

 
 

CAAGACGGAAACACGAGACA

      

PRDX6

GGCCGCATCCGTTTCCACGA

280

NM_214408

-0.29

0.998

1.95

 
 

ACTGGATGGCAAGGTCCCGACT

      

SOD

TCCATGTCCATCAGTTTGGA

250

NM_001190422

-0.27

0.998

1.88

 
 

CTGCCCAAGTCATCTGGTTT

      

GPX1

GGCACAACGGTGCGGGACTA AGGCGAAGAGCGGGTGAGCA

235

NM_214201

-0.29

0.998

1.96

 
  1. #Coefficient of determination of the standard curve. *The efficiency is determined by [10-slope].
  2. Abbreviations: RSP9 40S ribosomal protein S9-like, ATP5G1 ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1 (subunit 9), GSR glutathione reductase, ACTB actin, beta, SHAS2 hyaluronan synthase 2, HP haptoglobin, FGG fibrinogen γ, CFB complement factor B, CRP C-reactive protein, LBP lipopolysaccharide-binding protein, NQO1 NAD(P)H dehydrogenase, quinone 1, TXNRD1 thioredoxin reductase 1, UGT1A1 glucuronosyltransferase 1 family, and polypeptide A1; HO-1 heme oxygenase 1, PRDX6 peroxiredoxin 6, SOD superoxide dismutase, GPX1 glutathione peroxidase 1.
Back to article page

Acta Veterinaria Scandinavica

ISSN: 1751-0147