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Table 2 Average DNA fragmentation values associated with the centrifugation+extender treatments (means±SD)

From: The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders on the fragmentation dynamics of thawed equine sperm DNA

Treatment

sDFI 0

sDFI 4

sDFI 6

sDFI 24

B-C

16.36a±11,56

22.15b±11,58

27.01a±15,66

38.40b. c±13,23

B-1

13.23a±7,08

16.06a. b±5,64

27.07a±7,37

35.95a. b. c±10,38

B-2

11.86a±5,87

18.11a. b±7,32

23.13a±8,62

33.91a. b. c±14,50

I-C

14.61a±5,84

18.43a. b±7,17

23.51a±14,28

31.13a. b. c±15,59

I-1

14.52a±10,70

19.55a. b±15,61

20.81a±9,15

30.54a. b±16,94

I-2

13.69a±10,39

14.22a±8,73

17.81a±8,45

25.94a±10,24

G-C

17.01a±10,64

17.17a. b±7,19

23.69a±12,14

41.83c±19,39

G-1

12.75a±8.86

17.87a. b±9,06

24.44a±11,16

34.99a. b. c±8,76

G-2

11.96a±6,23

14.44a±8,82

22.44a±17,71

34.74a. b. c±15,32

  1. DNA fragmentation index values immediately after thawing (sDFI 0) and after 4 (sDFI 4), 6 (sDFI 6) and 24 h (sDFI 24) of incubation. Treatments: C,standard control centrifugation, 1, colloidal centrifugation Protocol 1, 2, colloidal centrifugation Protocol 2, B, Botu-Crio®, I, InraFreeze®, G, Gent®. Letters in superscript represent significant differences between extenders (p<0.05) at each time point.