The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders on the fragmentation dynamics of thawed equine sperm DNA

Table 4 Average DNA fragmentation values associated with the different centrifugation protocols (means±SD)

Centrifugation Protocol	sDFI 0	sDFI 4	sDFI 6	sDFI 24
Standard control centrifugation	21.50^b±9,54	24.31^b±8,97	24.74^a±13,91	37.12^a±16,56
Protocol 1	13.50^a±8,85	17.83^a.b±10,79	24.17^a±11,41	33.90^a±12,40
Protocol 2	12.50^a±7,66	15.59^a±8,35	21.19^a±12,41	31.53^a±13,86

DNA fragmentation index average values immediately after thawing (sDFI 0) and after 4 (sDFI 4), 6 (sDFI 6) and 24 h (sDFI 24) of incubation. Letters in superscript represent significant differences between extenders (p<0.05) at each time point.

ISSN: 1751-0147