From: Malignant peripheral nerve sheath tumor as a cause of chronic cardiac insufficiency in cattle
Antibody | Code no. | Clone | Dilution | Antigen retrieval |
---|---|---|---|---|
Monoclonal | ||||
Mouse anti-vimentinb | 180052 | V9 | 1:200 | 3 min/125°C, 0,01M citrate buffer pH 6.0 |
Mouse anti-human neuron-specific enolase (NSE) a | M 0873 | BBS/NC/VI-H14 | 1:100 | 3 min/125°C, 0,01M citrate buffer pH 6.0 |
Mouse anti-human cytokeratina | M3515 | AE1/AE3 | 1:80 | 3 min/125°C, 0,01M citrate buffer pH 6.0 |
Mouse anti-human desmina | M 0760 | D33 | 1:300 | Microwave (700w), 3x 5min,0,01M citrate buffer pH 6.0 |
Polyclonal | ||||
Rabbit anti-S100a | Z 0311 | 1:200 | 20 min/100°C, 0,01M citrate buffer pH 6.0 | |
Rabbit anti-glial fibrillary acidic protein (GFAP) a | Z 0334 | 1:500 | 10 min/100°C, Tris-EDTA buffer Ph 9,0 | |
Rabbit anti-bovine neurofilamentc | AHP245 | 1:500 | 10 min/37°C Trypsin 0,1% and Microwave (700w), 2 min, 0,01M citrate buffer Ph 6.0 | |
Rabbit anti-human Von Willebrand Factora | A0082 | 1:800 | 3 min/125°C, 0,01M citrate buffer Ph 6.0 |