Acta Veterinaria Scandinavica

Table 3 Statistics for the regression analyses

From: Impact of heparin and short term anesthesia on the quantification of cytokines in laboratory mouse plasma

	Linear regression					Exponential regression
Cytokine	R	R²	p	A $(\frac{pg \times \frac{1}{ml}}{µ})$	B $(\frac{pg}{ml})$	R	R²	p	K $(\frac{1}{µl})$	Y₀× 10⁻³ $(\frac{pg}{ml})$
IL-9	0.48	0.23	0.008	−163.46	7.7 × 10⁻³	0.39	0.15	0.035	−0.04	5.6
IL-10	0.40	0.16	0.027	−97.15	7.6 × 10⁻³	0.56	0.32	0.001	−0.02	7.7
IL-12p40	0.87	0.76	0.000	−317.36	12.0 × 10⁻³	0.87	0.76	0.000	−0.05	12.0
IL-12p70	0.71	0.50	0.000	−379.48	19.0 × 10⁻³	0.74	0.54	0.000	−0.03	20.0
IL-17A	0.38	0.15	0.037	−266.41	18.5 × 10⁻³	0.11	0.01	0.547	−0.01	12.7
Eotaxin	0.65	0.42	0.000	−438.07	18.0 × 10⁻³	0.74	0.55	0.000	−0.05	19.0
KC	0.83	0.70	0.000	−960.63	27.0 × 10⁻³	0.91	0.83	0.000	−0.16	26.0
MCP-1	0.91	0.83	0.000	−156.83	6.0 × 10⁻³	0.91	0.82	0.000	−0.05	6.4
MIP-1β	0.74	0.55	0.000	−278.96	11.0 × 10⁻³	0.47	0.22	0.009	−0.04	7.6
RANTES	0.72	0.52	0.000	−20.70	0.9 × 10⁻³	0.65	0.42	0.000	−0.04	0.8

For cytokines that were significantly different between serum and heparin, the data were analyzed for a correlation between the concentration of the analyte and the amount of heparin added; R, R², p-values, slopes (A) and intercepts (B) for the linear regressions and intercepts (y₀) and coefficients (K) for the exponential regressions are given. The calculations are based on the addition of 1 μl or 10 μl heparin (5,000 IU/ml). Where significant, the relationship between the specific cytokine concentration (pg/ml) and heparin (X) volume (μl) can be described as [Cyt] = B + A × X or $[Cyt] = y_{0} \times e^{(- K \times X)}$ .

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ISSN: 1751-0147

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