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Studies on Electrophoresis on Cellulose Acetate Membrane of Bovine Serum Proteins in Healthy Animals
Acta Veterinaria Scandinavica volume 10, pages 118–126 (1969)
Abstract
A method for rapid electrophoresis of serum proteins on a cellulose acetate membrane is described.
In order to experimentally identify the various larger globulin fractions in normal bovine serum, pure bovine y-globulin and a bovine attraction (Antitrombin III) were used.
These fractions were electrophoretically compared with bovine serum and the various fractions on the diagram determined.
A diluted veronal buffer with added Ga-acetate gave considerably better separation than the reported standard buffer.
In the last part of the experiment normal percentage values for the composition of the protein fractions in bovine serum were calculated by the method described. The material consisted of 50 cows of the N.R.F. breed. The values are compared with corresponding figures reported by other authors, and the results are discussed.
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Acknowledgment
Antitrombin III was supplied by Dr. U. Abildgaard, Ullevâl Hospital, Department IX, Oslo, who also gave valuable advice and help during the experimental work. The author owes him a great debt of gratitude.
The author would also like to thank Prof. dr. M. Braend, at present with the Faculty of Veterinary Science, University College, Nairobi, Kenya, for his valuable advice and guidance during the experiment.
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Ek, N. Studies on Electrophoresis on Cellulose Acetate Membrane of Bovine Serum Proteins in Healthy Animals. Acta Vet Scand 10, 118–126 (1969). https://doi.org/10.1186/BF03548283
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DOI: https://doi.org/10.1186/BF03548283