Fig. 3
From: Pathological findings in a Dachshund-cross dog with neuroaxonal dystrophy
Photomicrographs showing immunohistochemical reactivity of axonal spheroids. Transversal sections of thoracic spinal cord (gracilis and cuneatus fasciculi) show numerous spheroid immunohistochemically reactive (IR) for neurofilaments (a), tau (c), synaptophysin (e), ubiquitin (g), and LC3B (i) of thoracic tract of the spinal cord the NAD-affected puppy. As expected, single spheroids were identified in the healthy dog (b, d arrow head), while LC3B IR is observed in the glial cells of the affected and control dogs (i, j). Synapthophysin was also evident in the neuropil of the control (f). Avidin–biotin-peroxidase complex method with Mayer’s hematoxylin counterstain. Bar = 100 µm