Table 1 Technical details of the immunohistochemical examinations
From: Pathological findings in a Dachshund-cross dog with neuroaxonal dystrophy
Primary antibody | Host | Dilution | Antigen retrieval | Manufacturer (code no) |
|---|---|---|---|---|
Neurofilaments (NF-L) | Mouse | 1:800 | Citrate buffer 0.01 M pH 6.0 (3 × 5 min at 750 W) | Dako, Carpinteria, CA (M 0762) |
Synaptophysin | Mouse | 1:25 | Citrate buffer 0.01 M pH 6.0 (3 × 5 min at 750 W) | Dako, Carpinteria, CA (M 0776) |
Ubiquitin | Rabbit | 1:150 | None | Dako, Carpinteria, CA (Z0458) |
Tau | Rabbit | 1:100 | Citrate buffer 0.01 M pH 6.0 (3 × 5 min at 750 W) | Sigma-Aldrich, St. Louis, MO (T5530) |
Glial fibrillary acidic protein (GFAP) | Rabbit | 1:100 | None | BIOGENEX, Freemont, CA (932-020 M-4) |
LC3B | Rabbit | 1:800 | Citrate buffer 0.01 M pH 6.0 (3 × 5 min at 750 W) | Abcam, Cambridge, UK (Ab 48394) |