Table 3 Insulin concentrations used for in vitro experiments based on published literature
Insulin during IVM (µg/ml) | Insulin during IVC (µg/ml) | References | Main finding |
|---|---|---|---|
10 | 10 | [2] | No effect on BC rate |
5 (*) | – | [3] | No effect on BC rate nor cell number |
1 | – | [4] | No effect on BC rate, cell number elevated |
0.1/0.5/1/10 | – | [5] | 0.5–10 μg/ml stimulated development until morula |
5 (7, 12 or 22 h) | – | [6] | Difference as different co-factor |
– | 5 (¤) | [7] | BC rates similar to those obtained by serum addition |
– | 5 (¤) | [8] | Insulin alone no beneficial effect on BC rates |
– | 0.01 and 10 (®) | [9] | Insulin no effect on BC rate nor cell number but negative effect on apoptosis |
– | ED50 0.1 for granulosa cell mitogenesis ED50 0.01 for estradiol production ED50 for 0.1 for progesterone production ED50 0.2 for androgen production | [11] | ED50 depends on different aspect of insulin action |