Serum C-reactive protein concentrations in Nova Scotia Duck Tolling Retrievers with immune-mediated rheumatic disease
© The Author(s) 2017
Received: 1 September 2016
Accepted: 7 April 2017
Published: 17 April 2017
Nova Scotia Duck Tolling Retrievers (NSDTRs) are a dog breed often affected by immune-mediated rheumatic disease (IMRD), a disorder characterised by chronic stiffness and joint pain. Most, but not all, dogs with IMRD, have antinuclear antibodies (ANA), which are also commonly present in the autoimmune disease systemic lupus erythematosus (SLE). The clinical and diagnostic findings of IMRD indicate that it is an SLE-related disorder. C-reactive protein (CRP), an acute phase protein, is a quantitative marker of inflammation for many diseases and is used for diagnosing and monitoring systemic inflammation in both humans and dogs. However, in human SLE, CRP concentrations are often elevated but correlate poorly with disease activity; they can be low in individual patients with active disease. The aim of the study was to investigate CRP in a group of NSDTRs with the SLE-related disorder IMRD. The hypothesis was that CRP concentrations would be increased in dogs with IMRD compared to healthy dogs, but that the increase would be mild. Serum CRP concentrations were measured in 18 IMRD-affected NSDTRs and 19 healthy control NSDTRs using two different canine-specific CRP assays. Dogs with IMRD and ANA had higher CRP concentrations than the control dogs, but the concentrations were below the clinical decision limit for systemic inflammation for most of the IMRD dogs. These results indicate that CRP concentrations were increased in dogs with IMRD and ANA, but the increase was mild, similar to what has been observed in human SLE.
KeywordsC-reactive protein Canine Immune-mediated rheumatic disease Nova Scotia Duck Tolling Retriever Systemic lupus erythematosus
Nova Scotia Duck Tolling Retriever (NSDTR) dogs are, compared to other dog breeds, at increased risk of autoimmune disorders and particularly immune-mediated rheumatic disease (IMRD) [1, 2]. Dogs affected by IMRD have a clinical picture indicative of chronic non-erosive polyarthritis with stiffness and pain from multiple joints, sometimes in conjunction with other clinical manifestations. Most, but not all, IMRD cases display antinuclear antibodies (ANA) on indirect immunofluorescence (IIF) . Antinuclear antibodies and non-erosive polyarthritis are also commonly present in the systemic autoimmune inflammatory disease, systemic lupus erythematosus (SLE), in humans as well as in dogs [3–5] and IMRD is considered an SLE-related disorder [2, 6].
Measurement of C-reactive protein (CRP), an acute phase protein produced in the liver during systemic inflammation, is used for diagnosing and monitoring inflammatory diseases in both humans and dogs [7–9]. CRP is a quantitative marker of inflammation , and its measurement is used to monitor disease activity in dogs with immune-mediated polyarthritis [11, 12]. Despite being a reliable marker of systemic inflammation, CRP concentrations in human SLE correlate inconsistently with disease activity. CRP concentrations are often elevated, but individual patients with active disease can have low CRP concentrations [13, 14]. In the present study, we investigated the CRP concentrations in a group of NSDTRs with the SLE-related disorder IMRD. The hypothesis was that the serum CRP concentrations in these dogs would be higher than in the healthy controls, but that the elevation would be milder than in immune-mediated polyarthritis in other breeds [11, 15, 16].
Age, sex and ANA test result for the 37 dogs included in the study
ANA test result
For the current study, the serum samples were thawed and the serum CRP was measured in duplicate with a validated canine-specific immunoturbidimetric assay (Gentian cCRP, Gentian AS, Moss, Norway) , in a single run. For samples with CRP concentrations <6.8 mg/L, i.e., the assay’s limit of quantification (LOQ), re-analysis was performed in duplicate with a second, high-sensitivity canine-specific CRP assay (Gentian hsCRP)  on the same occasion. Analyses were performed on a fully automated, open-system clinical chemistry/immunoassay analyser (Abbott Architect c4000, Abbott Park, IL, USA). Statistical analyses were performed using software R V3.0.2. To compare the CRP concentrations between the three groups, Welch ANOVA was performed followed by pair-wise comparisons with Welch two-sample t test. Calculations were performed on log-transformed data. The significance level was set to P < 0.017 using Bonferroni adjustment for multiple testing. CRP concentrations <0.50 mg/L, corresponding to the LOQ of the hsCRP assay, were assigned the value 0.25 mg/L in the statistical analyses.
Despite the statistically significant difference in CRP concentration between the IMRD ANApos and healthy dogs, several of the IMRD dogs in both the ANApos and the ANAneg groups had concentrations similar to those of the healthy dogs. This resembles the CRP alterations in human SLE, where the CRP concentrations are often mildly elevated, but can also be low in individual patients with active disease . In most of the IMRD dogs, the increase in CRP concentration was less than in immune-mediated polyarthritis in other dog breeds [11, 15, 16], but higher than concentrations reported for e.g., canine osteoarthritis . A CRP of 20 mg/L has previously been established at our laboratory as the decision limit for clinical diagnosis of systemic inflammation. This decision limit was determined by performing an ROC curve analysis of CRP data from dogs with and without known systemic inflammatory disease (data not shown), similarly to what has been previously described . In the present study, most of the IMRD dogs had CRP concentrations below or just above the clinical decision limit. Measurement of CRP concentrations below the clinical decision limit is necessary to detect smaller changes between healthy and diseased dogs, as shown in the present study.
There was no significant difference between the IMRD ANAneg dogs and healthy controls, but two dogs had markedly elevated CRP concentrations (>100 mg/L). These two had typical clinical signs of IMRD, but the diagnosis of IMRD can be challenging, especially in the absence of ANA. It is also possible that these dogs suffered from disorders other than, or in addition to, IMRD that induced systemic inflammation, with resulting high CRP concentrations. The CRP concentrations in these two dogs were of similar magnitude as those reported in other studies of immune-meditated polyarthritis [11, 15, 16]. In a study by Hillström et al. , CRP was measured with the same assay as here and the median CRP concentration in dogs with immune-mediated arthritis was 118.0 mg/L, which is considerably higher than the medians in the present study. Unfortunately, direct comparison of CRP concentrations between other studies is not feasible because of the lack of standardization of CRP measurements.
Different veterinarians examined the dogs, which is a limitation of the study. Another limitation is that handling and storage of the samples were not standardised. However, CRP is stable at room temperature , and in freezer storage  and is unaffected by repeated freeze–thaw cycles . The results of the present study indicate that CRP concentrations increased in NSDTRs with IMRD, but the concentrations varied and were often below the clinical decision limit for systemic inflammation. Two of the individual IMRD ANAneg dogs had high CRP concentrations which might indicate that they suffered from concurrent or disorders other than IMRD. The CRP alterations in IMRD ANApos dogs are similar to those reported in humans with SLE . Whether CRP has a potential clinical value for diagnosing and monitoring IMRD in individual dogs, warrants further investigations.
- ANApos :
antinuclear antibody positive
- ANAneg :
antinuclear antibody negative
immune-mediated rheumatic disease
limit of quantification
Nova Scotia Duck Tolling Retriever
systemic lupus erythematosus
All the authors conceived and designed the study together. HDB, MK and HHA performed sampling and clinical evaluation of the dogs. AH performed the laboratory analyses and HDB the statistical analyses. HDB and AH drafted the manuscript. All authors participated in preparation of the manuscript. All authors read and approved the final manuscript.
Kerstin Lindblad-Toh is thanked for input on the manuscript.
HHA has been deputy editor of Acta Veterinaria Scandinavia, but has not in any way been involved in or interacted with the review process or editorial decision making. AH’s Ph.D. project, led by RH, at the Swedish University of Agricultural Sciences (SLU) was partly financed by Gentian AS, Moss, Norway, the company manufacturing the CRP assays in this study. The other authors declare that they have no competing interests.
Availability of data and materials
The dataset is available upon reasonable request.
Consent for publication
All dog owners gave their informed consent before participation.
Ethics approval and consent to participate
The study was approved by the Uppsala Animal Ethical Committee and Swedish Board of Agriculture (C417/12).
Financial support was received from The Swedish Research Council Formas project number 2011-1404, the Swedish Kennel Club and Agria Research Foundation, and the Eurostars project Mammalian CRP through Vinnova project no 2010-01777.
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