Table 1 Primers/probes used for PCR to detect Avibacterium paragallinarum, Mycoplasma gallisepticum and Gallid herpesvirus 1
Target organism | Oligo name | Sequence (5ʹ–3ʹ) with modifications | Refs |
|---|---|---|---|
A. paragallinarum | APG-Qf | GCAAAAGACTACCAGCAAGGATAAT | 20 |
APG-Qr | CCTTACCCAAATATAATGTTCCACATT | ||
APG-Pr | FAM-TCCTAGTTAGCATTATTGC-MGB | ||
M. gallisepticum | MG-Qf | GCTGGGTTGATTGTTGTTTCTT | 21 |
MG-Qr | TCTTCACGTTCTTGGATCATCAT | ||
MG-Pr | FAM-CTCTTSGGTTTAGGGATTGGGATTCCG-IBFQ | ||
Gallid herpesvirus 1 | IPC4 qPCR-F | CCCCACCCAGTAGAGGAC | In-house |
IPC4 qPCR-R | CGAGATACACGGAAGCTGATTT | ||
IPC4-Pr | FAM-CAGTCTTTGGTCGATGACCCGC-TAMRA |