Table 1 Immunohistochemistry protocols
Protocol | Human | Dog-adapted | Dog-adapted |
|---|---|---|---|
Target | Melan-A | Melan-A | PNL2 |
Antigen retrieval | - | EDTA buffer, pH9 | Sodium citrate |
Primary antibody | Melan-A clone 103, Dako (dilution 1:100) | Melan-A clone 103, Dako (dilution 1:50) | PNL2 clone sc-593,006, Santa Cruz (dilution 1:100) |
Secondary antibody | Envision Flex system labelled polymer anti-mouse | Envision + system-HRP labelled polymer anti-mouse | Envision + system-HRP labelled polymer anti-mouse |
Detection/ chromogen | DAB chromogen (Dako) | DAB chromogen (Dako) | DAB chromogen (Dako) |