Table 2 Impact of incubation time of agar-gels, sedimentation time in incubation jars, and addition of ice and 1% glucose to the incubation fluid and the agar on numbers of A. suum larvae (mean ± SD) recovered from liver tissue. All samples: One hour sedimentation in conical beakers, n = 4 unless something else is specified. No significant differences in worm counts between treatments were obtained.
Incubation time of agar-gels | |||||||
3 h | 5 h | ||||||
Sedimentation conditions in jars | 1 h+ice+ glucose | 1 h+ice | 1 h | 2 h+ice | 2 h | 1 h+ice | 1 h |
Larval count in sediment | 507 ± 81 (n = 3) | 607 ± 310 | 750 ± 145 (n = 3) | 657 ± 118 (n = 3) | 849 ± 30 | 749 ± 276 | 812 ± 18 |
Larval count * in supernatant | 4 | 0 | 6 | 7 | - | 13 | 4 |
% larvae in supernatant | <1 | 0 | <1 | <1 | - | 3 | <1 |