Antibacterial Effect of Bovine Lactoferrin Against Udder Pathogens

Table 1 Effect of Lf on bacterial growth in ISB culture, measured by lag time and maximum absorbance.

	Lag time (h)			Maximum absorbance
Bacteria	Mean	Min-max	SD	Mean	Min-max	SD
Escherichia coli (n = 5)
Control	3.8	3.0–4.0	0.45	0.91	0.70–1.05	0.15
0.67 mg/ml Lf	6.6	5–9	1.52	0.67 *	0.57–0.73	0.06
1.67 mg/ml Lf	9.0	5–15	4.1	0.52 *	0.25–0.70	0.18
2.67 mg/ml Lf	10.6	6.0–20.0	6.0	0.42 *	0.11–0.68	0.24
Staphylococcus aureus (n = 5)
Control	4.8	4.0–5.0	0.45	0.60	0.49–0.65	0.07
0.67 mg/ml Lf	7.2	5.0–11	2.4	0.55	0.42–0.69	0.11
1.67 mg/ml Lf	8.4 *	6.0–12	2.2	0.52	0.40–0.66	0.10
2.67 mg/ml Lf	8.4 *	6.0–12	2.2	0.49 *	0.40–0.64	0.10
Coagulase-negative staphylococci (n = 5)
Control	6.4	5.0–10	2.2	0.56	0.53–0.60	0.03
0.67 mg/ml Lf	12.2	6.0–20	7.2	0.36	0.10–0.56	0.23
1.67 mg/ml Lf	12.2	6.0–20	2.2	0.34	0.11–0.52	0.21
2.67 mg/ml Lf	12.4	7.0–20	7.0	0.34	0.10–0.55	0.21

Lag time = time from the beginning of incubation until the time-point when the absorbance began to increase; maximum absorbance = highest absorbance value measured during the 20-h incubation period.
Statistically significant difference when compared with negative control: * p ≤ 0.05

ISSN: 1751-0147