Table 2 Calcium Regaining Time (CRT) in min in cows administered one of 4 experimental diets. Each diet was given for 10 days, and the calcium homeostatic mechanism for each cow was challenged by a standardized intravenous 5% Na2EDTA infusion on day 11. Intravenous infusion was continued steadily until the cow went involuntarily recumbent. Whole blood free calcium was monitored cow side during spontaneous recovery from the induced hypocalcaemia. The first blood sample was collected at recumbence. Subsequent blood samples were collected hourly until a whole blood free calcium concentration above 1.00 mmol/l was regained. CRT at 1.00 mmol/l was then extrapolated graphically from plots.