Detection and identification by PCR of Clostridium chauvoei in clinical isolates, bovine faeces and substrates from biogas plant

Table 2 Comparisons of Clostridium chauvoei detected by different methods

Sample	Material	Culture followed by biochemical identification	PCR on culture colonies	PCR on muscle piece or blood	PCR on meat juice	PCR on muscle piece macerated in sterile physiological saline
AN 3656/05	blood	+	+	-	nd	nd
AN 165/06	muscle	+	+	+	-	+
AN 1106/06	muscle	+	+	-	-	+
AN 1717/06	muscle	+	+	-	-	-
AN 1818/06	muscle	-	-	-	-	-
AN 2091/06	muscle	-	+	-	-	-
B 16476/07	muscle	-	+	+	-	-
B 17520/07	muscle	-	-	-	-	-
B 19034/07	muscle	-	-	-	-	-
P 2500/07	muscle	+	+	nd	nd	nd
P 2855/07	muscle	-	+	-	+	+
P 4822/07	muscle	-	-	-	-	-
P 2446/08	muscle	-	+	-	-	-
B 29245/08	muscle	-	+	-	-	+
B 32286/08	muscle	-	-	-	-	-
P 3086/08	muscle	+	+	-	-	-
P 3240/08	muscle	-	-	-	-	-
B 41901/08	muscle	-	-	-	-	-
B 41902/08	muscle	-	-	-	-	-
B 43014/08	muscle	-	-	-	-	-
B 43531/08	muscle	-	-	-	-	+
P 3837/08	muscle	-	-	+	-	+
B 46320/08	muscle	+	+	-	-	-
P 4324/08	muscle	-	+	-	+	-
P 4325/08	muscle	+	+	-	-	-
Summary		8+/25	14+/25	3+/24	2+/23	6+/23

nd = Not determined
Comparisons of Clostridium chauvoei detected by culture followed by biochemical identification culture followed by PCR and direct PCR on muscle tissue from clinical samples of blackleg.

ISSN: 1751-0147