Detection and identification by PCR of Clostridium chauvoei in clinical isolates, bovine faeces and substrates from biogas plant

Table 3 Comparison between muscle tissue samples and swab samples

Sample	Culture followed by biochemical identification	Summary of PCR results from Table 2	1 day	3 days	6 days
AN 165/06	+	+	-	-	-
AN 1106/06	+	+	+	+	+
AN 1717/06	+	+	-	+	-
AN 2091/06	-	+	-	-	-
B 16476/07	-	+	+	+	+
B 17520/07	-	-	-	-	-
B 19034/07	-	-	-	-	-
P 2855/07	-	+	+	+	+
P 4822/07	-	-	-	-	-
P 2446/08	-	+	-	-	-
B 29245/08	-	+	+	+	+
B 32286/08	-	-	-	-	-
P 3086/08	+	+	+	+	+
P 3240/08	-	-	-	-	-
B 41901/08	-	-	-	-	nd
B 41902/08	-	-	-	-	nd
B 43014/08	-	-	-	-	nd
B 43531/08	-	+	-	+	-
P 3837/08	-	+	-	-	+
B 46320/08	+	+	+	+	+
P 4324/08	-	+	+	-	+
P 4325/08	+	+	+	-	+
Summary	6+/22	14+/22	8+/22	8+/22	9+/19

nd = Not determined
Muscle tissue samples from suspected cases of blackleg. Clostridium chauvoei were analysed by culture and biochemical identification, culture and PCR and swab samples from the same muscle samples were analysed by culture and PCR. The swabs were used to simulate a postal service simulation by keeping them on the bench for 1, 3 and 6 days.

ISSN: 1751-0147