Table 1 Major characteristics and modifications of the three commercial bovine ELISA kits used to test reindeer for alphaherpesvirus antibodies in this study.

SVANOVA – A

Indirect

BoHV-1 unknown antigen in one well and cells on another.

Rabbit anti-reindeer antibody

Streptavidin-POD + OPD

450 nm

OD _S = (OD _IBR - OD _CONTROL ) Sample is positive if OD _SAMPLE >0

SYNBIOTICS – B

Blocking

BoHV-1 gB antigen

2 monoclonal antibodies (Mabs) anti-gB/peroxidase

Peroxidase system

450 nm + 620 nm (for correction)

Validation rules: %P = [(OD_N - OD_P)/OD_N] ×100 > 80% and OD_N>0,500

Sample is positive if: %S = [(OD_N - OD_S)/(OD_N - OD_P)] ×100 > 60

Sample is doubtful if: 45<%S<60

LSI – C

Blocking

BoHV-1 gB antigen

1 monoclonal antibody anti-gB/HRP labelled

Horseradish Peroxidase

450 nm + 620 nm (for correction)

Validation rules: %P = [(OD_N - OD_P)/OD_N] ×100 > 70% and OD_N>0,700

Sample is positive if: %S = [(OD_N - OD_S)/(OD_N - OD_P)] ×100 > 50

Sample is doubtful if: 45<%S<50