Evaluation of three commercial bovine ELISA kits for detection of antibodies against Alphaherpesviruses in reindeer (Rangifer tarandus tarandus)

Table 3 Virus neutralization test (VNT) on reindeer sera tested in this study.

ELISA Kits results			No. of samples		Neutralizing antibody titre [min and max titre] average	Difference between CvHV-2 and BoHV-1 in dilution steps
A	B	C		CvHV-2	BoHV-1	[min – max] average
+	+	+	62	[1:2–1:256] 1:45	[0–16] 1:3	[1-5] 3.2
-	±	-	3	[0]	[0]	0
-	+	±	1	[0]	[0]	0
-	+	+	1	[1:2]	[0]	1
-	-	-	87	[0]	[0]	0
+	+	+	Bovine + control	[1:2]	[1:32]	4
+	+	+	Reindeer + control	[1:128]	[1:16]	3

Neutralizing titres are expressed as the reciprocal of the highest serum dilution that completely prevented a cytopathic effect (CPE). For each virus neutralization, the maximum and minimum titres obtained in each group of samples are presented. Samples were grouped according to the results they obtained in the different ELISA kits (+ for positive; ± for doubtful; – for negative). [0] represents the absence of neutralization. The difference between the neutralizing titres against CvHV-2 and BoHV-1 is presented in dilution steps differences.

ISSN: 1751-0147