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Figure 1 | Acta Veterinaria Scandinavica

Figure 1

From: Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection

Figure 1

Visualisation of Lawsonia intracellularis in tissue of inoculated intestinal loops. Immunohistochemistry/haematoxylin stain of Lawsonia intracellularis in intestinal tissue; arrows point at immunopositive red stained L. intracellularis. A and B: Bacteria overlying ileal epithelium 6 h post inoculation (PI). A) Vaccine derived inoculum. B) Wild-type derived inoculum. In both (A) and (B) close interactions between bacteria and enterocytes is not found. Low level oedema seen as distended central lacteal (A) (asterisk). C and D: Solitary L. intracellularis bacteria in intimate contact with the brush border of enterocytes 6 h PI. C) Vaccine derived inoculum in jejunal loop. D) Cell culture propagated vaccine in ileal loop. E and F: Solitary intracellular L. intracellularis bacteria in villus enterocytes 6 h PI. E) Vaccine derived inoculum in jejunal loop. F) Cell culture propagated vaccine in ileal loop. Insert in (E) shows a higher magnification of the area with the intracellular bacterium. Bars: 10 μm.

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