Application of a pig ligated intestinal loop model for early Lawsonia intracellularis infection

Table 1 Overview of types- and concentrations of inocula used in each ligated intestinal loops.

Loop No.	Inoculation type	Inoculum concentration
1	Wild-type	4-6 × 10⁸ bacteria/ml
2	Live vaccine	3-5 × 10⁶ bacteria/ml
3	Propagated live vaccine	2-8 × 10⁷ bacteria/ml
4	Negative control	Mock inoculum
5	Wild-type	4-6 × 10⁸ bacteria/ml
6	Live vaccine	3- 5 × 10⁶ bacteria/ml
7	Propagated live vaccine	2-8 × 10⁷ bacteria/ml
8	Wild-type	4-6 × 10⁸ bacteria/ml
9	Live vaccine	3-5 × 10⁶ bacteria/ml
10	Propagated live vaccine	2-8 × 10⁷ bacteria/ml

Ligation of ten loops (1-10) was done in the ileum and the jejunum, respectively. All three types of inocula applied were exposed for 1, 3 and 6 h, while only at 6 h a negative control was included (loop No. 4). The concentration of Lawsonia intracellularis in the inocula is shown in the table; 5 ml of inoculum were used for each loop.

ISSN: 1751-0147