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Table 1 Sequences of primers designed for AMP PCR in combination with Hpa II restriction enzyme treatment

From: Different DNA methylation patterns detected by the Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) technique among various cell types of bulls

Primer Sequence (5'-3') Primer Sequence (5'-3')
1 TGGACCGG TG 16 AAGACCGG GA
2 ACCCGG TCAC 17 TCCCGG TGAG
3 AACCCGG GAA 18 GAATCCGG CA
4 TTCCCGG GTT 19 ACCCGG AAAC
5 TTTGCCCGG T 20 TGCCGG TTCA
6 CCCGG CATAA 21 AGCCGG GTAA
7 CACCCGG ATG 22 CCCGG AAGAG
8 TCAGTCCGG G 23 CTACCGG CAC
9 TGCCGG CTTG 24 ACCTCCGG TC
10 CCCCGG TAAC 25 CTCCGG ATCA
11 CAGTGCCGG T 26 TTTCCGG GAG
12 ACCGG CTTGT 27 AGGCCGG TCA
13 GTCCGG AGTG 28 CAACCGG TCT
14 ACACCGG AAC 29 CCGCCGG TAA
15 CCCGG ATGGT 30 TCCGG GACTC
  1. Four bases in bold letters are the Hpa II enzyme recognition sequence