From: Evaluation of multicomponent recombinant vaccines against Actinobacillus pleuropneumoniae in mice
Genes | Primer sequences (5'-3') | Annealing temperature | Size of PCR product (bp) |
---|---|---|---|
apxIA | Forward: GCGGGATCC AACTCTCAGCTCGATAG | 55°C | 2520 |
 | Reverse: GATGCGTCGAC AGCAGATTGTGTTAAAT |  |  |
apxIIA | Forward: GCGGGATCC ATGTCAAAAATCACTT | 54°C | 2721 |
 | Reverse: GCGAATTC AGCGGCTCTAGCTAAT |  |  |
apxIIIA | Forward: ACGGGATCC TGGTCAAGCATGTTAG | 52°C | 3114 |
 | Reverse: ATGCGTCGAC TGCTCTAGCTAGGTTACC |  |  |
apxIVA | Forward: GCCGAATTC CGCGCCTATATCTGG | 54°C | 2553 |
 | Reverse: ATGCGTCGAC CCCTTCGAATTGTTTC |  |  |
Omp | Forward: GGAATTC ACGCCTAAGGTTGATAT | 53°C | 984 |
 | Reverse: GGTCGAC CTTTATCTTCTTTTGTTG |  |  |
Apfa | Forward: GGGCGAATTC ATGCAAAAACTAAGT | 53°C | 444 |
 | Reverse: TATGGTCGAC TGATGCGCAGAAAT |  |  |