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Table 1 Primers, sequences and PCR conditions used for the amplification of apxIA, apxIIA, apxIIIA, apxIVA, omp and apfa from Actinobacillus pleuropneumoniae

From: Evaluation of multicomponent recombinant vaccines against Actinobacillus pleuropneumoniae in mice

Genes Primer sequences (5'-3') Annealing temperature Size of PCR product (bp)
apxIA Forward: GCGGGATCC AACTCTCAGCTCGATAG 55°C 2520
  Reverse: GATGCGTCGAC AGCAGATTGTGTTAAAT   
apxIIA Forward: GCGGGATCC ATGTCAAAAATCACTT 54°C 2721
  Reverse: GCGAATTC AGCGGCTCTAGCTAAT   
apxIIIA Forward: ACGGGATCC TGGTCAAGCATGTTAG 52°C 3114
  Reverse: ATGCGTCGAC TGCTCTAGCTAGGTTACC   
apxIVA Forward: GCCGAATTC CGCGCCTATATCTGG 54°C 2553
  Reverse: ATGCGTCGAC CCCTTCGAATTGTTTC   
Omp Forward: GGAATTC ACGCCTAAGGTTGATAT 53°C 984
  Reverse: GGTCGAC CTTTATCTTCTTTTGTTG   
Apfa Forward: GGGCGAATTC ATGCAAAAACTAAGT 53°C 444
  Reverse: TATGGTCGAC TGATGCGCAGAAAT   
  1. Note:
  2. Bam H I site: Underlined; Eco R I site: Italic; Sa I I site: Bold; PCRs were run for 30 cycles.