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Table 1 Primers, sequences and PCR conditions used for the amplification of apxIA, apxIIA, apxIIIA, apxIVA, omp and apfa from Actinobacillus pleuropneumoniae

From: Evaluation of multicomponent recombinant vaccines against Actinobacillus pleuropneumoniae in mice

Genes

Primer sequences (5'-3')

Annealing

temperature

Size of PCR

product (bp)

apxIA

Forward: GCGGGATCC AACTCTCAGCTCGATAG

55°C

2520

 

Reverse: GATGCGTCGAC AGCAGATTGTGTTAAAT

  

apxIIA

Forward: GCGGGATCC ATGTCAAAAATCACTT

54°C

2721

 

Reverse: GCGAATTC AGCGGCTCTAGCTAAT

  

apxIIIA

Forward: ACGGGATCC TGGTCAAGCATGTTAG

52°C

3114

 

Reverse: ATGCGTCGAC TGCTCTAGCTAGGTTACC

  

apxIVA

Forward: GCCGAATTC CGCGCCTATATCTGG

54°C

2553

 

Reverse: ATGCGTCGAC CCCTTCGAATTGTTTC

  

Omp

Forward: GGAATTC ACGCCTAAGGTTGATAT

53°C

984

 

Reverse: GGTCGAC CTTTATCTTCTTTTGTTG

  

Apfa

Forward: GGGCGAATTC ATGCAAAAACTAAGT

53°C

444

 

Reverse: TATGGTCGAC TGATGCGCAGAAAT

  
  1. Note:
  2. Bam H I site: Underlined; Eco R I site: Italic; Sa I I site: Bold; PCRs were run for 30 cycles.