Table 1 Primers, PCR profiles, PCR product sizes and restriction enzymes (RE) used for identification of bovine leukocyte adhesion deficiency (BLAD), deficiency of uridine monophosphate synthase (DUMPS), complex vertebral malformation (CVM), bovine citrullinaemia (BC) and factor XI deficiency (FIXD)

BLAD

Newly designed

F: 5' GAATAGGCATCCTGCATCATATCCACCA 3'

R: 5' CTTGGGGTTTCAGGGGAAGATGGAGTAG 3'

94°C

03 m

33

cycle

357 bp

Taq I

94°C

30 s

65°C

30 s

72°C

30 s

72°C

05 m

DUMPS

[13]

F: 5' GCAAATGGCTGAAGAACATTCTG 3'

R: 5' GCTTCTAACTGAACTCCTCGAGT 3'

94°C

05 m

40

cycle

108 bp

Ava I

94°C

60 s

58°C

60 s

72°C

90 s

72°C

05 m

CVM

Newly designed

F: 5' CAGATTCTCAAGAGCTTAATTCTA 3'

R: 5' TATTTGCAACAACAAGCAGTT 3'

94°C

05 m

35

cycle

281 bp

-

94°C

45 s

52°C

45 s

72°C

60 s

72°C

10 m

BC

[25]

F: 5' GGCCAGGGACCGTGTTCATTGAGGACATC 3'

R: 5' TTCCTGGGACCCCGTGAGACACATACTTG 3'

94°C

03 m

35

cycle

198 bp

Ava II

94°C

30 s

57°C

30 s

72°C

30 s

72°C

10 m

FXID

[32]

F: 5' CCCACTGGCTAGGAATCGTT 3'

R: 5' CAAGGCAATGTCATATCCAC 3'

95°C

10 m

34

cycle

320 bp

-

95°C

30 s

55°C

60 s

72°C

30 s

72°C

10 m