Western blot analysis of Cx43 protein expression levels in epididymides of control and flutamide-exposed boars. Representative Western blot shows a band at approximately 43 kDa in epididymal samples of control and flutamide-treated groups (prenatal exposure - GD20, GD80; postnatal exposure - PD2, PD90). Lane H indicates pig heart used as the positive control. Lane M contains prestained protein standards; the molecular weights in kDa are indicated on the left. Relative Cx43 levels in the caput, corpus and cauda epididymides (A, B, C) expressed as arbitrary units. Data obtained from three separate analyses is expressed as mean ± SD (n = 3 for each group). Significant differences from control values are denoted as *p < 0.05, **p < 0.01.