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Table 1 Positive results obtained by analysis of faeces and sera from 176 pigs in 24 herds collected during two previous studies, A and B, and analysed by nested PCR and by two serological methods, ELISA and IFAT.

From: Evaluation of a blocking ELISA for the detection of antibodies against Lawsonia intracellularis in pig sera

 

PCR

ELISA (PI > 30)*

ELISA (PI > 35)*

IFAT

Study A. Pigs from poor performance herds

    

No of positive animals (%)

29 (44)

37 (56)

34 (52)

47 (71)

No of positive herds (%)

8 (89)

9 (100)

9 (100)

9 (100)

Study A. Pigs from good performance herds

    

No of positive animals (%)

0

0

0

3 (25)

No of positive herds (%)

0

0

0

3 (75)

Study B. Pigs with diarrhoea and poor performance

    

No of positive animals (%)

24 (22)

46 (42)

25 (23)

20 (18)

No of positive herds (%)

5 (45)

9 (82)

5 (45)

4 (36)

  1. *In the blocking ELISA, two cut-off values (PI 30 and PI 35, respectively) were evaluated.