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During laser stimulation
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Tail flick
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The tail is flicked at least 5 cm to either side. A new event is recorded after a complete cycle of tail movement [12].
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Tail pressing
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The cow is pressing the central part of the tail against the base of the udder [13].
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Muscle twitch
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Contraction of single muscle group. A new event is recorded after a pause of at least 5 seconds.
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Type of initial response toward stimulation
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Kicking
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The hoof is thrusted against the floor or withdrawn at high speed [12].
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Lifting leg
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The hoof is lifted from the floor in a calm manner [12].
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Stepping
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The leg is moved, but the hoof is not lifted from the floor.
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No response
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The leg is not moved within the temporal limits of the test [12].
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Behavior during 30 s after exposure
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Moving hind leg
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At least one hind leg is moved. The hoof does not have to be lifted from the floor.
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Licking leg
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The cow licks the exposed hind leg or attempts to do so by turning the head against the hind leg.
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Licking body
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The cow licks other body parts than the hind legs.
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Lying down
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The cow changes posture from standing to lying.
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