Table 4 The activity of nucleotidases of brain ventricular system under optimal pH conditions.
From: Some aspects of purinergic signaling in the ventricular system of porcine brain
Incubation mixture | Ependymal ecto-enzymes activity (nmol × min-1 × (cm2)-1)* | ||||
|---|---|---|---|---|---|
Ion | Inhibitor | ATP = > ADP | ATP = > AMP | ADP = > AMP | ADP = > ATP |
| Â | Â | pH 7.5 | pH 8.5 | pH 7.5 | pH 6.5 |
Ca2+ | Control (without inhibitor) | 19.2 ± 1.1 | 20.1 ± 3.6 | 17.8 ± 3.5 | 11.3 ± 1.2 |
|  | Suramine | 6.9 ± 1.2 | 17.2 ± 2.8 | 7.6 ± 1.3 | 11.2 ± 1.7 |
|  | Ap5A | 16.5 ± 2.3 | 5.2 ± 0.9 | 17.7 ± 2.1 | 4.2 ± 1.0 |
Mg2+ | Control (without inhibitor) | 19.4 ± 1.9 | 20.4 ± 2.4 | 16.7 ± 2.0 | 18.5 ± 1.9 |
|  | Suramine | 7.1 ± 2.0 | 19.4 ± 2.5 | 6.9 ± 1.5 | 18.2 ± 1.9 |
|  | Ap5A | 21.0 ± 3.5 | 5.1 ± 1.1 | 16.5 ± 0.9 | 4.8 ± 0.9 |
Incubation mixture | CSF exo-enzymes activity (nmol × min -1 × mg -1 )* | ||||
Ion | Inhibitor | ATP = > ADP | ATP = > AMP | ADP = > AMP | ADP = > ATP |
| Â | Â | pH 7.0 | pH 8.0 | pH 7.5 | pH 6.5 |
Ca2+ | Control (without inhibitor) | 21.1 ± 2.6 | 4.0 ± 0.8 | 18.1 ± 2.1 | 18.5 ± 2.1 |
|  | Suramine | 10.9 ± 0.9 | 2.7 ± 0.9 | 11.2 ± 1.1 | 17.6 ± 1.2 |
|  | Ap5A | 11.9 ± 1.2 | 2.8 ± 1.2 | 14.9 ± 0.8 | 4.6 ± 0.7 |
Mg2+ | Control (without inhibitor) | 23.0 ± 3.5 | 5.9 ± 2.3 | 25 ± 1.9 | 24.5 ± 2.1 |
|  | Suramine | 13.2 ± 2.3 | 3.8 ± 1.6 | 15.2 ± 2.3 | 23.5 ± 3.2 |
|  | Ap5A | 11.2 ± 1.6 | 4.4 ± 1.6 | 9.1 ± 0.1 | 5.3 ± 0.8 |