Results of Sephacryl S-200 HR column (5.0 × 100 cm) gel filtration of the CM Fr-1 peak in Figure 1. The main peak (Fr. A) proved to contain chicken CA-III. Starting buffer: 0.05 M Tris-HCl (pH 8.0) containing 0.5 M NaCl. Absorbance values were read at 280 nm. Flow rate: 20 mL/h.