Figure 7

Standard curves for chicken CA-III concentrations generated by use of the competive ELISA specific for chicken CA-III. Typical standard curves were plotted for a range of CA-III solutions (2-400 ng/mL). The coefficients of variation (CV) for these solutions were as follows: 400 ng/mL, CV = 4.3%; 200 ng/mL, CV = 4.8%; 100 ng/mL, CV = 2.4%; 50 ng/mL, CV = 6.8%; 25 ng/mL, CV = 3.6%; 12 ng/mL, CV = 3.1%; 6 ng/mL, CV = 2.4%. Values were obtained by using a spectroscope at 405 nm.