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Table 1 Outcome of different subculturing steps

From: Yersinia enterocolitica in sheep - a high frequency of biotype 1A

   Number of strains  
Subculturing steps Days of incubation API 20E identified Ye BT1A
1. PSB (i) 0 (3h) 3 0
2. TSBY (ii) 1 7 4
3. TSBY+KOH-treatment (ii) 1 17 8
4. TSBY+KOH-treatment (ii) 2 13 9
5. PSB+MRB (i) 8+4 18 8
6. PSB (i) 21 22 5
Total   80 34
  1. The number of isolates identified as Yersinia enterocolitica (Ye) by API 20E and biotyped as 1A (BT1A) found at different subculturing (CIN) steps during the culture procedures applied.
  2. (i), (ii) refers to the different pathways, shown in Figure 1. PSB, Phosphate-buffered saline containing 2% sorbitol and 0.15% bile salts; TSBY, Tryptone-soya broth supplemented with yeast; KOH, Potassium hydroxide solution; MRB, Modified-Rappaport-broth.