Figure 1

A maximum likelihood (ML) phylogenetic tree constructed from an alignment of the 5S IGS sequence from the unknown sample (12-17-97b) with the top ten* sequence entries from a Blast search. Tree inference was constructed by using nearest neighbor interchange. Nodes indicate support after bootstrap replicates (500). Sequences from the top hits were from the following organisms: Toxocara canis (TCU65503), Ascaris lumbricoides (M27961.1), Chandlerella quiscali (HM641830), Foleyella furcata ( AJ250988), Mansonella ozzardi (JF412308), Onchocercidae cf. Mansonella ozzardi (JF412312-13, JF412315-16). The closest match (indicated by the shortest horizontal distance of the branches in the tree) with respect to our sample, was sequence TCU 65503 from Toxocara canis, which was only 79.8% similar. Gaps and deletions in the alignment were treated as complete deletion and this only allowed for 262 nucleotide sites to be analysed. *(one sequence from Litomosoides sigmodontis LSU31639 was omitted as this was only 160 bp long and made the data in the analysis significantly less informative).