An evaluation of the ability of Dichelobacter nodosus to survive in soil

Table 1 Results based on plate wash of cultures from the four groups of samples, presented on the different test days with Ct-values and observation of Dichelobacter nodosus -suspect colonies

	Soil 5°C		Soil and hoof powder 5°C		Soil 15°C		Soil and hoof powder 15°C
Day	Ct-value^a	Colonies^b	Ct-value	Colonies	Ct-value	Colonies	Ct-value	Colonies
4	17.19	Y	17.53	Y	18.83	N	18.47	Y
	17.28	Y	18.15	Y	17.12	Y	15.52	Y
7	18.08	Y	18.52	Y	34.02	Y	22.68	N
	18.64	N	16.91	Y	34.08	N	22.02	N
11	21.60	N	19.42	Y	33.34	N	31.15	N
	25.15	N	18.24	Y	33.97	N	32.13	N
14	24.04	Y	16.51	Y	35.87	N	31.77	N
	22.50	N	18.95	Y	30.87	N	45	N
17	29.95	N	19.98	Y	45	N	29.88	N
	25.10	Y	17.31	Y	45	N	30.64	N
	25.45	N	18.16	N	45	N	29.26	N
21	45	Y	24.92	Y	45	N	32.39	N
	45	Y	34.51	Y	45	N	31.65	N
	26.41	N	45	Y	45	N	33.07	N
24	36.57	Y	32.25	N	45	N	36.11	N
	34.23	N	19.27	N	45	N	31.87	N
	35.19	N	25.45	Y	45	N	31.56	N
28	45	N	36.07	N	45	N	31.63	N
	34.83	N	31.26	N	45	N	30.71	N
	45	N	32.57	N	45	N	33.00	N

^a: All Ct-values presented are for the 1:10 diluted samples. On day 4–14 samples were analyzed in duplicates whereas triplicates were used from day 17 to 28.
^b: Observed D. nodosus-suspect colonies on TASH agar plates (Yes/No).

ISSN: 1751-0147