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Table 1 Oligonucleotides used as primers for multiplex PCR in the present study

From: Phenotypic and genotypic identification of streptococci and related bacteria isolated from bovine intramammary infections

Bacterium target gene (protein)

Primer

Sequence 5′ - 3′

Amplicon size (bp)

Streptococcus uberis

GSub-S

TGA TTC CGA CTA CTA CGC TAG AT

723

pauA (plasminogen activator A)

GSub-AS

ATA CTT TGA GTT TCA CCG AGT TC

 

Streptococcus dysgalactiae

GSdys-S

GTG CAA CTG CAT CAC TAT GAG

279

16S rRNA

GSdys-AS

CGT CAC ATG GTG GAT TTT C

 

Streptococcus agalactiae

GSag-S

ATT GAT AAC GAC GGT GTT ACT GT

487

sklA3 (fibrinogen binding protein)

GSag-AS

CAT AGT AGC GTT CTG TAA TGA TGT C

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