Phenotypic and genotypic identification of streptococci and related bacteria isolated from bovine intramammary infections

Table 2 Evaluation of single phenotypic identification tests for Gram-positive, catalase-negative cocci

Bacterium	n	α H¹	CAMP¹	ESC¹	KM¹	NACL¹	INU¹	HIP¹	LAP¹	PYR¹
Streptococcus uberis	58	3%	9%	100%	8%^a	0%^a	100%^a	92%^a	100%^a	68%^a
Streptococcus dysgalactiae	20	100%	0%	0%	0%^b	0%^b	0%^b	0%^b	100%^b	0%^b
Streptococcus agalactiae	9	0%	100%	0%	0%	0%	0%	100%	100%	0%
Viridans streptococci	17	88%	0%	53%	47%	0%	24%	24%	88%	6%
Enterococcus spp	3	1/3	0/3	3/3	3/3	1/3	2/3	1/3	2/3	1/3
Aerococcus viridans	30	87%	0%	100%	3%	73%	13%	100%	3%	27%
Lactococcus garvieae	12	67%	0%	100%	0%	0%	17%	92%	100%	83%
Lactococcus lactis	2	0/2	0/2	2/2	1/2	0/2	2/2	2/2	2/2	1/2

All strains were isolated from milk of cows with intrammamary infection and previously identified by 16S rRNA sequencing. Data is expressed as percentage (%) of strains showing a positive reaction in the corresponding phenotypic tests.
¹Alpha-hemolysis (α H); CAMP reaction (CAMP); esculin hydrolysis (ESC); growth on kanamycin esculin azide agar (KM); growth on sodium chloride agar (NACL); inulin fermentation (INU); hippurate hydrolysis (HIP); leucine aminopeptidase activity (LAP); pyrrolidonyl peptidase activity (PYR).
^aFor these analyses, 25 strains of Streptococcus uberis were used.
^bFor these analyses, 13 strains of Streptococcus dysgalactiae were used.

ISSN: 1751-0147