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Table 3 Combination of phenotypic tests for identification of Gram-positive, catalase-negative cocci

From: Phenotypic and genotypic identification of streptococci and related bacteria isolated from bovine intramammary infections

Bacteria

Test pattern1

Strain characteristics

Probability

Number/pattern

Streptococcus uberis

– – + − − + + + +

Typical

92%

14/25

 

– + + − − + + + +

CAMP-positive

99%

1/25

 

– – + − − + + + −

PYR-negative

96%

7/25

Streptococcus dysgalactiae

+ − − – – – – + −

Typical

90%

13/13

Streptococcus agalactiae

– + − − – – + + −

Typical

100%

9/9

Viridans streptococci

+ − + − − – – + −

Typical

88%

2/17

 

+ − − – – – – + −

ESC-negative

10%

2/17

 

+ − + + − − – + −

KM-positive

100%

1/17

Aerococcus viridans

+ − + − + − + − −

Typical

100%

11/30

 

+ − + − − – + − −

NACL-negative

96%

5/30

 

+ − + − + − + − +

PYR-positive

100%

4/30

 

– – + − + − + − −

α H-negative

100%

2/30

Lactococcus garvieae

+ − + − − – + + +

Typical

99%

4/12

 

– – + − − – + + +

α H-negative

97%

3/12

 

+ − + − − – + + −

PYR-negative

66%

2/12

  1. All strains were isolated from milk of cows with intramammary infection. For each species and test, the outcomes were merged. If >50% of the strains showed a positive or negative result, “+” for positive or “-” for negative was attributed, respectively. Each value was then considered as the typical outcome of a variable for a particular bacterial species. For each species, a typical pattern was obtained by joining the typical outcomes of each variable. The probability was then calculated that a strain exhibiting the pattern belongs to the indicated species.
  2. 1The included tests are indicated in the following order: alpha-hemolysis; CAMP reaction; esculin hydrolysis; growth on kanamycin esculin azide agar; growth on sodium chloride agar; inulin fermentation; hippurate hydrolysis; leucine aminopeptidase activity; pyrrolidonyl peptidase activity.