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Table 2 Primary and secondary Abs used for immunohistological identification/localization and morphometric quantification of CD45+lymphoid cells residing ileal mucosa of 9-week-old pigs (N = 2) following 5 weeks after the treatment with a single dose of 10 mL of POE-POP copolymer

From: Effect of polyoxyethylene and polyoxypropylene nonionic block copolymers on performance and recruitment of immune cell subsets in weaned pigs

Abs

Targeted marker

Isotype

Clone/conjugate

Cellular/molecular specificity

Origin

Primary mAb

CD45RA

IgG1

MIL13

Subset of immunologically naive lymphoid cells

AbD Serotec, Kidlington, Oxford, UK

SecondaryAbs

Mouse IgG

IgG

IgG:HRP

Rabbit Ab against mouse IgG

Abcam, Cambridge, UK

 

Goat IgG

IgG

IgG:HRP

Goat Ab against rabbit IgG