Figure 3
From: Factors influencing the cell adhesion and invasion capacity of Mycoplasma gallisepticum
Detection of fibronectin in cultures of R low and R high . The occurrence of fibronectin in whole washed cells and cell lysates of M. gallisepticum strains Rlow and Rhigh was analyzed in Western blot (A) and dot blot (B) assays by use of a fibronectin-specific antibody. Purified fibronectin (Fn) as well as the reference culture S. pneumoniae DSM20566 (+) were used as positive controls, while E. coli DH10B (−) served as a negative control. A high molecular weight band comigrating with purified fibronectin could be detected in cell lysates of Rlow and Rhigh(A). Sizes of the molecular weight markers (MWM) are given in kDa next to the arrows. Dot blot analyses (B) indicated the binding of fibronectin to the bacterial cell surface of both M. gallisepticum strains, Rlow and Rhigh.