Table 1 Primary antibodies and immunohistochemical protocols applied in the study

Monoclonal

Mouse anti-vimentin^b

180052

V9

1:200

3 min/125°C, 0,01M citrate buffer pH 6.0

Mouse anti-human neuron-specific enolase (NSE) ^a

M 0873

BBS/NC/VI-H14

1:100

3 min/125°C, 0,01M citrate buffer pH 6.0

Mouse anti-human cytokeratin^a

M3515

AE1/AE3

1:80

3 min/125°C, 0,01M citrate buffer pH 6.0

Mouse anti-human desmin^a

M 0760

D33

1:300

Microwave (700w), 3x 5min,0,01M citrate buffer pH 6.0

Polyclonal

Rabbit anti-S100^a

Z 0311

1:200

20 min/100°C, 0,01M citrate buffer pH 6.0

Rabbit anti-glial fibrillary acidic protein (GFAP) ^a

Z 0334

1:500

10 min/100°C, Tris-EDTA buffer Ph 9,0

Rabbit anti-bovine neurofilament^c

AHP245

1:500

10 min/37°C Trypsin 0,1% and Microwave (700w), 2 min, 0,01M citrate buffer Ph 6.0

Rabbit anti-human Von Willebrand Factor^a

A0082

1:800

3 min/125°C, 0,01M citrate buffer Ph 6.0