Skip to main content

Table 1 Primary antibodies and immunohistochemical protocols applied in the study

From: Malignant peripheral nerve sheath tumor as a cause of chronic cardiac insufficiency in cattle

Antibody Code no. Clone Dilution Antigen retrieval
Monoclonal
Mouse anti-vimentinb 180052 V9 1:200 3 min/125°C, 0,01M citrate buffer pH 6.0
Mouse anti-human neuron-specific enolase (NSE) a M 0873 BBS/NC/VI-H14 1:100 3 min/125°C, 0,01M citrate buffer pH 6.0
Mouse anti-human cytokeratina M3515 AE1/AE3 1:80 3 min/125°C, 0,01M citrate buffer pH 6.0
Mouse anti-human desmina M 0760 D33 1:300 Microwave (700w), 3x 5min,0,01M citrate buffer pH 6.0
Polyclonal
Rabbit anti-S100a Z 0311   1:200 20 min/100°C, 0,01M citrate buffer pH 6.0
Rabbit anti-glial fibrillary acidic protein (GFAP) a Z 0334   1:500 10 min/100°C, Tris-EDTA buffer Ph 9,0
Rabbit anti-bovine neurofilamentc AHP245   1:500 10 min/37°C Trypsin 0,1% and Microwave (700w), 2 min, 0,01M citrate buffer Ph 6.0
Rabbit anti-human Von Willebrand Factora A0082   1:800 3 min/125°C, 0,01M citrate buffer Ph 6.0
  1. a Dako Denmark A/S, Glostrup, Denmark.
  2. bZymed Laboratories Inc., San Francisco, USA.
  3. cAbDSerotec, Oxford, UK.