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Figure 1 | Acta Veterinaria Scandinavica

Figure 1

From: The presence of heat-labile factors interfering with binding analysis of fibrinogen with ferritin in horse plasma

Figure 1

Binding of horse fibrinogen and fibrinogen in horse plasma to holoferritin and apoferritin. Plasma from 4 horses was diluted 100-fold with buffer A and heated at 60°C for 30 min, followed by centrifugation (14,000 × g, 15 min). Aliquots (100 μL) of purified horse fibrinogen (1 μg/well) (A) or resulting supernatant from heat-treated plasma (B) were added to wells of holo- or apo-ferritin-coated plate (1 pmol/well each). Fibrinogen bound to the wells was detected as using a goat anti-human fibrinogen antibody and an ALP-labeled rabbit anti-goat IgG antibody. Each data represents mean ± SD of four determinations or the average data from each horse. *: P < 0.01 compared to holoferritin.

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