An enzyme-linked immunosorbent assay for detection of avian influenza virus subtypes H5 and H7 antibodies

Table 5 Comparison of detection of seroconversion of H5 and H7 antibodies with ELISA and HI test

H5/H7	Number of animals	ELISA	HI
Week 0	60/42	0/3.1	0/0
Week 1	41/27	54/59	2.4/11
Week 2	41/27	88/78	39/63
Week 3	41/27	98/100	93/100

The percentage of chickens which seroconverted in H5-ELISA (cut-off = 20 Inh%) and HI test (cut-off = 16), when sera were tested against heterologous N-antigen (H5N7 sera tested by ELISA plates coated with inactivated H5N2 virus and H5N2 sera tested by inactivated H5N7 antigen). After the slash are shown the percentage of chickens which seroconverted in H7-ELISA (cut-off = 20 Inh%) and HI test (cut-off = 16). Sera were tested in ELISA against heterologous N-antigen (H7N1 sera tested by ELISA plates coated with inactivated H7N7 virus and H7N7 sera tested by inactivated H7N1 virus), while all sera in HI test were tested against H7N1 inactivated virus.

ISSN: 1751-0147