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Table 1 Statistics of Experiment 1

From: Impact of heparin and short term anesthesia on the quantification of cytokines in laboratory mouse plasma

Cytokine Distr. N (NO_ANE/ANE) Anova (p) Fried Wilc.-sign. rank Mann Whit. Conclusion
S H E Sample ANE As. p S-H S-E H-E As. p Sample (p) ANE (p)
IL-1α Log 5/7 5/7 5/6 0.018 0.113       S < E (0.033) NS
IL-1β Log 5/7 5/7 5/7 0.392 0.772       NS NS
IL-2 NND 6/7 6/7 6/6    0.482     0.418 NS NS
IL-6 NND 6/7 6/7 6/6    0.081     0,167 NS NS
IL-9 Log 5/7 5/7 5/7 0.440 0.963       NS NS
IL-12p40 Log 5/7 5/7 5/7 0.192 0.124       NS NS
IL-12p70 NND 6/7 6/6 6/6    0.241     0.280 NS NS
IL-13 NND 6/7 6/7 6/6    0.746     0.824 NS NS
IL-17A* NND 6/7 6/7 6/6    0.013 0.143 0.915 0.070 0.218 H < E < S* NS
G-CSF Log 5/7 5/7 5/7 0.194 0.016       NS NO_ANE > ANE
GM-CSF ND 5/7 5/7 5/7 0.174 0.923       NS NS
IFN-γ ND 5/4 5/4 5/4 0.495 0.700       NS NS
KC NND 6/7 6/7 6/6    0.002 0.699 0.003 0.015 0.929 S < E, H < E NS
MCP-1 Log 4/7 4/7 4/7 0.015 0.736       H < E (0.002) NS
MIP-1α ND 5/7 5/7 5/7 0.769 0.777       NS NS
MIP-1β NND 6/7 6/7 6/6    0.789     0.670 NS NS
RANTES NND 6/7 6/7 6/6    0.001 0.130 0.005 0.230 0.614 S < E NS
TNF-α NND 6/7 6/7 6/6    0.138     0.669 NS NS
  1. Shown are the data distributions of the 18 measured that were above the detection limit cytokines for the sample types (Sample) serum (S), heparin-plasma (H) and EDTA-plasma (E) and for the effect of isoflurane anesthesia (ANE): normal distribution (ND), log-normal distribution (Log) and non-normal distribution (NND), and the number (N) of samples in each group (NO_ANE /ANE). P-values and asymptotic significances of a repeated measured ANOVA, Friedman test, Wilcoxon-signed rank test and Mann Whitney test are given with significant differences highlighted in bold type. The conclusion states, for each cytokine, the difference between sample types, or whether no significant differences (NS) were found. Where significant differences were found the sample types are listed in increasing order based on their mean values. *IL-17A showed an overall significant difference in the Friedman test but not in the Wilcoxon-signed rank post hoc test, why the mean rank of the anticoagulant groups are listed in increasing order. Five cytokines (IL-3, IL-4, IL-5, IL-10 and Eotaxin) were below the detection limit and were therefore not included in the analysis.