Table 1 Antibodies used for multichannel immunofluorescence microscopy
Antigen | Clone | Supplier/Product ID | Isotype | Reactivity |
|---|---|---|---|---|
FMDV VP1 | 6HC4 | in-house | IgG2b | FMDV serotype A capsid [[16]] |
FMDV VP1 | 10GA4 | in-house | IgG3 | FMDV serotype O capsid [[17]] |
Actin | Acta-1 | Lifespan Biosciences/ | Rabbit | Actin |
CD163 | 2A10-11 | Serotec/MCA2311GA | IgG1 | Monocytes, macrophages |
CD172a | 74-22-15a | Wasington State University/PG2031 | IgG2b | Monocytes, macrophages, granulocytes |
CD44 | P-BAG40A | Wasington State University/PBOV2037 | IgG3 | Leukocytes |
CD8α | MIL-12 | Serotec/MCA1223 | IgG2a | CTLs and NK-cells |
CD3 | SP7 | Abcam/ab16669 | Rabbit | T-cells |
CD21 | LT21 | Abcam/ab1090 | IgG1 | B-cells |