Antibody designation | Type or isotype | Specificity | Dilution | Antigen retrieval | References/source |
---|---|---|---|---|---|
D490 | Rabbit hyperimmune serum | M. bovis | 1:1800 | 0.25% trypsina (37°C, 60 min) | [26] |
mAbb pool | Mouse IgG and IgM | M. bovis | 1:2000 | 0.25% trypsin (37°C, 60 min) | Chemicon |
1A1 | Mouse IgG1 | M. bovis VspA, C, and other Vspsc | 1:200 | 0.25% trypsin (37°C, 60 min) | [27] |
1E5 | Mouse IgM | M. bovis VspA, B, C | undiluted | 0.25% trypsin (37°C, 60 min) | |
Anti-CD3 | Rabbit IgG | CD3+ T lymphocytes | 1:300 | Demasking solution Gd (95°C, | DakoCytomation |
BAQ44A | Mouse IgM | B lymphocytes | 1:100 | 20 min) 0.01 M citric buffer (microwave 95°C, 10 min) | VMRD; [23] |
Anti-MRP8 (S100A8) | Mouse IgG | Human MRP8e | 1:600 | Pronase Ef (37°C, 20 min) [26], | BMA Biomedicals; [30] |
Anti-MRP14 (S100A9) | Mouse IgG | Human MRP14g | 1:8000 | 0.01 M citrate buffer (microwave 95°C, 10 min) | BMA Biomedicals; [30] |
TAL.1B5 | Mouse IgG1 | α-chain of human leukocyte antigen (HLA-DR) | 1:4000 | 0.01 M citrate buffer (microwave 95°C, 15 min) | DakoCytomation |
Anti-iNOS/NOS II | Rabbit IgG | iNOS | 1:50 | Saponinef (37°C, 20 min) | |
Anti-NT | Rabbit IgG | NT | 1:100 | Pronase E (37°C, 20 min) | Millipore GmbH |