Day | N positive | Mean Cqa(SD) | Mean log CFU/g (SD) |
---|
2 | 3 (of 3) | 35.3 (±2.13) | 3.8 (±0.6) |
3 | 1b (of 3) | 38.5 (±1.01) | 2.9 (±0.7) |
4 | 1c (of 3) | 39.0 (±0.60) | 2.6 (±2.2) |
5 | 0 (of 3) | ND | ND |
8 | 0 (of 3) | ND | ND |
- aMean Cq represents the mean of the positively quantified samples. Each sample was run in three qPCR replicates. The corresponding mean log CFU was calculated from the standard curve.
- Additionally, 2b (or 1c) other samples were positive after TPGY-enrichment (Cq not shown).
- qPCR, quantitative real-time PCR.
- Cq, quantification cycle.
- SD, Standard deviation.
- CFU, Colony-forming units.
- ND, Not detected; less than 2 replicates per sample yielded Cq values <40.