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Table 3 Comparison of sensitivity of the in-house Schmallenberg virus ELISA (A) and a commercial SBV ELISA (B) versus virus neutralization test (VNT)

From: Development and evaluation of an indirect enzyme-linked immunosorbent assay for serological detection of Schmallenberg virus antibodies in ruminants using whole virus antigen

Dilution of sera

Sera number

611

612

615

616

 

VNT

A

B

VNT

A

B

VNT

A

B

VNT

A

B

1:1

1:32

98

96

1:64

76

189

1:64

95

120

1:64

81

64

1:4

1:16

34

53

1:16

34

213

1:32

49

102

1:16

36

25

1:8

1:8

18

34

1:8

22

132

1:16

31

61

1:8

24

21

1:16

1:4

9

17

1:4

15

86

1:4

16

35

<1:4

11

11

1:32

<1:4

3

8

<1:4

8

43

<1:4

7

16

1:4

6

−4

1:64

<1:4

3

4

<1:4

4

23

<1:4

5

9

<1:4

3

11

  1. Four out of the positive sera from France (see Table 1); serially diluted 1:4 to 1:64 in negative serum were analyzed in accordance with the protocol of each test. The sera and sera dilutions where further diluted 1:100 in ELISA A, 1:10 in ELISA B and serially diluted 1:4 to 1:256 in VNT. Bold-figures indicate positive results, under-lined figures doubtful results. Thresholds: VNT titer ≥1:8 = positive. ELISA A; S/P% ≥15 = positive, ELISA B; S/P ≥50-60 = doubtful, >60 = positive.