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Table 3 MANOVA and specific ANOVA showing influence of Iberian ibex captivity (t0 ibexes were in the wild, t1-t5 in the enclosure), and treatment (t2 and t3, before and after treatment, respectively) on physiological parameters (i.e., haematological parameters and acute phase protein concentration) and anti- C.pseudotuberculosis antibody presence (i.e., Optical Density (O.D.) obtained by means of ELISA) as a canonical response

From: Management of a caseous lymphadenitis outbreak in a new Iberian ibex (Capra pyrenaica) stock reservoir

MANOVA Pillai’s trace F-value P-value
Early captivity (t0 vs t1) 0.974 15.793 0.001
Long-term captivity (t0 vs t1-t5) 0.818 10.284 3.468e-08
Treatment (t2 vs t3) 0.735 1.979 0.141
ANOVA Mean ± SE (Min-Max) F-value P-value
Early captivity    
IgG (O.D.)   37.993 6.352e-06
t0 0.22 ± 0.02 (0.16-0.4)
t1 0.5 ± 0.04 (0.22-0.7)
Leukocytes (×103 cells/μl)   5.338 0.032
t0 11.2 ± 1.33 (5.56-21.98)
t1 15.16 ± 0.86 (12.11-19.95)
Platelets (×103 cells/μl)   6.652 0.018
t0 260.82 ± 52.41 (46–563)
t1 470.55 ± 58.3 (109–784)
Monocytes (×103 cells/μl)   7.370 0.014
t0 2.16 ± 0.58 (0.6-6.2)
t1 0.59 ± 0.15 (0.2-2)
Eosinphils (×103 cells/μl)   5.085 0.036
t0 3.13 ± 0.67 (1–8.4)
t1 5.08 ± 0.72 (1.8-10)
Basophils (×103 cells/μl)   10.604 0.004
t0 1.76 ± 0.37 (0.6-5.2)
t1 0.59 ± 0.05 (0.4-1)
Total Protein (g/dl)   15.952 0.001
t0 7.1 ± 0.13 (6.4-7.9)
t1 8.31 ± 0.27 (7–9.6)
Long-term captivity    
IgG (O.D.)   10.432 0.002
t0 0.23 ± 0.03 (0.17-0.4)
t1-t5 0.48 ± 0.03 (0.196-1.37)
Basophils (×103 cells/μl)   56.797 1.644e-09
t0 1.38 ± 0.13 (0.6-1.7)
t1-t5 0.61 ± 0.04 (0.2-1.2)
Treatment    
Haemoglobin (g/dl)   4.689 0.041
t2 10.46 ± 0.46 (7.7-13.2)
t3 11.92 ± 0.49 (8.6-13.6)
PCV (%)   5.061 0.034
t2 33.25 ± 1.54 (24.5-42.7)
t3 38.33 ± 1.65 (27.4-44.8)
  1. ANOVA values illustrate statistically significant parameters associated with captivity and treatment effect.