Table 3 MANOVA and specific ANOVA showing influence of Iberian ibex captivity (t0 ibexes were in the wild, t1-t5 in the enclosure), and treatment (t2 and t3, before and after treatment, respectively) on physiological parameters (i.e., haematological parameters and acute phase protein concentration) and anti- C.pseudotuberculosis antibody presence (i.e., Optical Density (O.D.) obtained by means of ELISA) as a canonical response
MANOVA | Pillai’s trace | F-value | P-value |
|---|---|---|---|
Early captivity (t0 vs t1) | 0.974 | 15.793 | 0.001 |
Long-term captivity (t0 vs t1-t5) | 0.818 | 10.284 | 3.468e-08 |
Treatment (t2 vs t3) | 0.735 | 1.979 | 0.141 |
ANOVA | Mean ± SE (Min-Max) | F-value | P-value |
Early captivity | |||
IgG (O.D.) | 37.993 | 6.352e-06 | |
t0 | 0.22 ± 0.02 (0.16-0.4) | ||
t1 | 0.5 ± 0.04 (0.22-0.7) | ||
Leukocytes (×103 cells/μl) | 5.338 | 0.032 | |
t0 | 11.2 ± 1.33 (5.56-21.98) | ||
t1 | 15.16 ± 0.86 (12.11-19.95) | ||
Platelets (×103 cells/μl) | 6.652 | 0.018 | |
t0 | 260.82 ± 52.41 (46–563) | ||
t1 | 470.55 ± 58.3 (109–784) | ||
Monocytes (×103 cells/μl) | 7.370 | 0.014 | |
t0 | 2.16 ± 0.58 (0.6-6.2) | ||
t1 | 0.59 ± 0.15 (0.2-2) | ||
Eosinphils (×103 cells/μl) | 5.085 | 0.036 | |
t0 | 3.13 ± 0.67 (1–8.4) | ||
t1 | 5.08 ± 0.72 (1.8-10) | ||
Basophils (×103 cells/μl) | 10.604 | 0.004 | |
t0 | 1.76 ± 0.37 (0.6-5.2) | ||
t1 | 0.59 ± 0.05 (0.4-1) | ||
Total Protein (g/dl) | 15.952 | 0.001 | |
t0 | 7.1 ± 0.13 (6.4-7.9) | ||
t1 | 8.31 ± 0.27 (7–9.6) | ||
Long-term captivity | |||
IgG (O.D.) | 10.432 | 0.002 | |
t0 | 0.23 ± 0.03 (0.17-0.4) | ||
t1-t5 | 0.48 ± 0.03 (0.196-1.37) | ||
Basophils (×103 cells/μl) | 56.797 | 1.644e-09 | |
t0 | 1.38 ± 0.13 (0.6-1.7) | ||
t1-t5 | 0.61 ± 0.04 (0.2-1.2) | ||
Treatment | |||
Haemoglobin (g/dl) | 4.689 | 0.041 | |
t2 | 10.46 ± 0.46 (7.7-13.2) | ||
t3 | 11.92 ± 0.49 (8.6-13.6) | ||
PCV (%) | 5.061 | 0.034 | |
t2 | 33.25 ± 1.54 (24.5-42.7) | ||
t3 | 38.33 ± 1.65 (27.4-44.8) |